17+ a modular cloning toolkit for genome editing in plants
The three panels shown come from different parts of the same DNA gel Additional file 4. The modular cloning kit presented in the study enables quick and facile assembly of DNA constructs for genome editing applications in plants and is an addition to previously.
Pdf A Modular Cloning Toolkit For Genome Editing In Plants
The âœpNCâ vector 11 system represents a high-throughput toolkit that can facilitate the large-scale analysis of plant 12 functional genomics.
. M is the DNA marker. Results Here we present an expanded cloning toolkit that contains 103 modules encoding a variety of CRISPRCas-based nucleases and their corresponding guide RNA. We believe the presented CRISPRCas toolkit is a great resource that will contribute towards wider adoption of the CRISPRCas genome editing technology and.
Here we describe the method of assembling CRISPRCas DNA constructs using the modular cloning MoClo for deployment in cereal species. NTC is the no template control - A modular cloning toolkit for genome. For this purpose we use a cloning toolkit.
A Modular Cloning Toolkit for Genome Editing in Plants Florian Hahn1 Andrey Korolev12 Laura Sanjurjo Loures1 and Vladimir Nekrasov1 The Dawn of the Crispr-Free Genome Editing Era.
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